Image analysis as PAT-Tool for use in extrusion-based bioprinting

The technology of bioprinting is arousing a growing interest in biopharmaceutical research and industry. In order to accelerate process development the field bioprinting, image-based analysis methods are non-invasive, time- cost-saving tools which useable for printer characterization, bioink printability evaluation, optimization. Image processing can also be used study reproducibility, since reliable production important transition from industrial application, more precisely clinical studies. This revolves around establishment an automated line method applications enables easy comparison 3D-printed lines. Diverse rheological properties bioinks printing affect geometry resulting object. represents simple geometry, where influence parameters directly apparent. Therefore, was developed on basis image recognition. At first, tested several substances such as Nivea®, pure colored Kolliphor solutions, two commercially available hydrogel formulations bioinks. These Biogelx™-ink-RGD by Biogelx Cellink® Bioink Cellink. examination limitations showed that transparent materials Kolliphor-based solutions cannot analyzed with whereas opaque Nivea® both analyzed. course investigate shrinkage behavior bionks. With help tool, demonstrated thus, time could identified critical parameter.